Study on Quality Control of Polygonum Capitatum from GAP Planting Base by HPLC Characteristic Spectrum
10.3969/j.issn.1005-5304.2014.08.020
- VernacularTitle:HPLC特征图谱对GAP基地产头花蓼药材品质控制研究
- Author:
Yu XIE
;
Lili ZHANG
;
Liang XU
;
Liyan ZHANG
;
Jingwen TANG
;
Mei PAN
- Publication Type:Journal Article
- Keywords:
Polygonum Capitatum;
HPLC;
specific chromatogram
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(8):70-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC characteristic spectrum of Polygonum Capitatum from GAP planting base, and provide reference for the overall quality control of Polygonum Capitatum. Methods HPLC analysis was performed on a DiamonsiL C18 chromatographic column (250 mm× 4.6 mm, 5μm) with the eluting system of gradient consisted of methanol and 0.2% H3PO4. The flow rate was 1.0 mL/min. The column temperature was maintained at 25℃ and the detection wavelength was at 254 nm.Results HPLC characteristic spectrum of Polygonum Capitatum samples from 10 different areas was established, which contained 11 common peaks, and the similarities of comparative results were over 90%.Conclusion The established method can be used to determine the characteristic spectrum of Polygonum Capitatum and can provide a basis for the overall quality control and quality standards of Polygonum Capitatum.
