Effects of Panax Notoginseng Saponins on Expression of ZO-1 of Brain Microvascular Endothelial Cells Caused by Aβ42

VernacularTitle:三七总皂苷对β-淀粉样蛋白42损伤脑微血管内皮细胞ZO-1表达的影响
Author: Likun YANG ; Lulu MANA ; Haiyan ZHU ; Tao MA ; Yikun SUN ; Yizhou ZHAO ; Yonghong GAO
Publication Type:Journal Article
Keywords: Panax Notoginseng Saponins; Alzheimer’s disease; β-amyloid42; ZO-1
From: Chinese Journal of Information on Traditional Chinese Medicine 2014;(8):57-59
CountryChina
Language:Chinese
Abstract: Objective To study the effects of Panax Notoginseng Saponins (PNS) on the damaged expression of ZO-1 of brain microvascular endothelial cells caused by Aβ42.MethodsBrain microvascular endothelial cells were divided into normal control group, model group, PNS low-, medium- and high concentration groups. They were incubated for 24h in 5% CO2 incubator at 37℃ . Then cell vitality was detected by MTT colorimetric method and ZO-1 protein expression was tested by Western blot.Results Stimulation of Aβ42 reduced the activity of microvascular endothelial cells (P<0.01) and suppressed the expression of ZO-1 protein (P<0.01). Compared with the model group, the activity of microvascular endothelial cells of PNS groups, especially the high and medium dose groups (P<0.05), and increased the ZO-1 protein expression. Conclusion PNS can partly recover the barrier function of blood brain barrier through inhibiting the decrease of the activity of microvascular endothelial cells caused by Aβ42.