Orthogonal Experiment Study on Extraction Process of Ginkgo Leaves
10.3969/j.issn.1005-5304.2014.08.023
- VernacularTitle:银杏叶提取工艺正交试验研究
- Author:
Jianbiao YAO
;
Jianfang WANG
;
Xuming WU
;
Huihui JIN
;
Zhongliang CHEN
- Publication Type:Journal Article
- Keywords:
ginkgo leaves;
total flavonol glycosides;
terpene lactones;
transfer rate;
orthogonal experiment
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(8):78-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the best extraction process of ginkgo leaves with the total transfer rate of total flavonol glycosides and terpene lactones as the index.Methods The effects of ethanol concentration, solid-liquid ratio and extraction time on extraction process were investigated by orthogonal design method, and the contents of total flavonol glycosides and terpene lactones were detected by HPLC to calculate transfer rate.Results The optimum extraction conditions were as follows:85% ethanol refluxing and extracting for three times;the first time extracting with five-fold amount of solvent (V/W) for 3 hours;the last two times with three-fold solvent (V/W) for 2 hours.Conclusion This extraction process has the advantages of simplicity of operator, reason, energy conservation, high efficiency, and is suitable for industrial production.
