Immune response of purified Plasmodium falciparum antigen on human pe-ripheral blood T lymphocytes in vitro
- VernacularTitle:恶性疟原虫抗原对健康人外周血T细胞免疫功能的影响
- Author:
Li CHEN
;
Li TAO
;
Qian LI
;
Zhiyong TAO
;
Qiang FANG
;
Hui XIA
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum;
Antigen;
Immunological function;
T lymphocyte;
IL-4;
IFN-γ
- From:
Chinese Journal of Schistosomiasis Control
2014;(4):410-414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of the purified Plasmodium falciparum(P. f)antigen on the T lymphocytes of the peripheral blood of healthy people in vitro. Methods The peripheral blood mononuclear cells(PBMCs)isolated from healthy donators were cultured with purified P. f antigen(5μg/ml)and normal RBC(nRBC)antigen(5μg/ml),in addition, the group in which only IL-2 added was set as a negative control. After cultured for 12 d,the corresponding antigens were used to re-stimulated the activated T cells in the two stimulated groups for 20 h. Then the cells were collected,the proliferations of the T cells labeled by CFSE were analyzed,and the secretion condition of IL-4 and IFN-γof the cells were detected by flow cytome-try. Results After amplification,the proliferative index(PI )of CD4+T cells that were stimulated with P. f antigen was signifi-cantly higher than those in the nRBC antigen group and the negative control group(both P<0.05),but there was no significant difference between the PIs ofγδT cells in the above three groups(P>0.05). Meanwhile,the percentages of the CD4+T cells se-creting IL-4+in the P. f antigen group was significantly higher than those in the other two groups(both P<0.05),while the per-centages of T cells secreting IFN-γin the 3 groups had no significant difference(P>0.05). Conclusion The purified P. f anti-gen could effectively stimulate the proliferation of CD4+T cells in peripheral blood of healthy people,and the latter can play a role in immunoregulation through secreting IL-4 preferentially.
