Investigation of the expression and DNA binding activity of signal transducer and activator of tran-scription 5 after interleukin 2 therapy for recurrent genital herpes
- VernacularTitle:白细胞介素2治疗对复发性生殖器疱疹患者信号转导和转录激活因子5信号调控的研究
- Author:
Guangzheng SUN
;
Xueyi ZHENG
- Publication Type:Journal Article
- Keywords:
Herpes genital;
Interleukin 2;
Signal transducer and activator of transcription 5;
T cell
- From:
Journal of Chinese Physician
2010;(z1):19-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of phosphorylation of signal transducer and activa -tor of transcription 5 (STAT5) protein and the DNA-binding activity of STAT5 after interleukin 2 therapy for genital herpes .Methods Six patients with recurrent genital herpes were included in this study .CD4 +T cells from patients who underwent interleukin 2 therapy were analyzed for changes in STAT 5 signaling.None had been treated with corticosteroid and immunosuppressive agents .Phosphorylation of STAT 5 was detected in the T cells using Western blot analysis .Electrophoretic mobility shift assay ( EMSA) was carried out to detect the DNA-biding activity of STAT5.Results The expressions of phosphorylated STAT 5 in T cells de-rived from patients with genital herpes 28 days after interleukin treatment were 1.8~2.7 fold to that of be-fore the treatment was given .STAT5 DNA-binding activity in T cells derived form patients with genital her-pes who had received the treatment was 2.3~3.4 fold increased compared to that of before the initiating of interleukin 2 treatment.The differences between the before and after interleukin 2 treatment were statistical-ly significant( t =10.6, P <0.05).Conclusion This study indicates that STAT5 may be an important signaling mediator of interleukin 2 therapies , and that STAT5 activation may predict response to this treat-ment .
