The effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231
10.3969/j.issn.1007-3969.2014.07.007
- VernacularTitle:miR-340对乳腺癌MDA-MB231细胞增殖和凋亡的影响
- Author:
Qianwen OUYANG
;
Yali CAO
- Publication Type:Journal Article
- Keywords:
Breast cancer cell MDA-MB231;
miR-340;
Proliferation;
Apoptosis
- From:
China Oncology
2014;(7):517-520
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:MicroRNA-340 (miR-340) has been demonstrated to play a role of negative regulation in many kinds of tumor, however, there are few reports about the relationship between miR-340 in proliferation and apoptosis of breast cancer cell. This study was aimed to explore the effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231. Methods: The pre-miR-340 or anti-miR-340 were transiently transfected into breast cancer cell MDA-MB231 with LipofectamineTM2000. miR-340 level was detected by RT-PCR. The Western blot was performed to detect the protein level of cleaved-caspase-3. The inhibition rate of cell proliferation was evaluated by MTT assay. The cell apoptosis was studied by lfow cytometry. Results:The pre-miR-340 facilitated the expression of miR-340 in MDA-MB231 cells. The pre-miR-340 enhanced the protein level of cleaved-caspase-3, inhibited the proliferation of MDA-MB231 cells and increased its apoptosis. On the contrary, the expression of miR-340 was inhibited by anti-miR-340 in MDA-MB231 cells. The protein level of cleaved-caspase-3 was reduced after the anti-miR-340-transfected MDA-MB231 cells. Anti-miR-340 promoted the proliferation of MDA-MB231 cells. Decreased apoptosis of MDA-MB231 cells was observed by lfow cytometry. Conclusion:The overexpression of miR-340 can effectively inhibit the proliferation and increase the apoptosis of MDA-MB231 cells, which may be explained by up-regulating of protein cleaved-caspase-3 level.
