Antimicrobial resistance characteristics and mechanisms of imipenem-re-sistant Pseudomonas aeruginosa
10.3969/j.issn.1671-9638.2014.07.001
- VernacularTitle:耐亚胺培南铜绿假单胞菌耐药特征及其耐药机制的初步研究
- Author:
Guowei DONG
;
Jie GUO
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
imipenem;
carbapenemase;
outer membrane protein;
drug-resistant gene;
drug resistance,microbial;
multidrug resistance
- From:
Chinese Journal of Infection Control
2014;(7):385-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate antimicrobial resistance characteristics and mechanisms of imipenem-resistant Pseudomonas aeruginosa (P .aeruginosa)isolated from clinical specimens.Methods Bacterial strains were identi-fied by BD Phoenix 100 automatic microbial analysis system,antimicrobial susceptibility testing was performed by Kirby-Bauer method;Carbapenemase genes (IMP ,VIM ,OXA,GES )and outer membrane protein gene oprD2 were detected by polymerase chain reaction.Results Resistant rates of imipenem-resistant P .aeruginosa strains to ami-kacin was the lowest (8.33%);resistant rates to gentamicin and tobramycin were<20%;resistant rates to the third and fourth generation cephalosporins,aztreonam,meropenem,compound sulfamethoxazole and minocycline were all>60%,and all strains were resistant to ampicillin/sulbactam.The positive rate of OXA-17 gene was 2.78%(n=1 ),deletion rate of oprD2 was 38.89%,the other drug-resistant genes were not detected.Conclusion Except aminoglycosides,resistance of imipenem-resistant P .aeruginosa to other antimicrobial agents is serious;resistance of P .aeruginosa to imipenem may be due to lacking of OprD2 and production of carbapenemases.
