Cloning,expression and immuno-protection analysis of a gene encoding tro-ponin T of Schistosoma japonicum(SjTnT)
- VernacularTitle:日本血吸虫肌钙蛋白T（SjTnT）基因克隆表达及免疫保护效果评估
- Author:
Xinzhuo WANG
;
Yang HONG
;
Hongxiao HAN
;
Changjian LI
;
Xiaodan CAO
;
Ke LU
;
Hao LI
;
Chuangang ZHU
;
Zhiqiang FU
;
Jiaojiao LIN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Troponin T;
Clone;
Expression;
Immuno-protection;
Efficacy evaluation
- From:
Chinese Journal of Schistosomiasis Control
2014;(4):394-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone cDNA encoding troponin T of Schistosoma japonicum(SjTnT),and evaluate the protective efficacy induced by recombinant SjTnT in BALB/c mice against S. japonicum challenge infection. Methods The SjTnT gene was amplified from 28-day-schistosome cDNAs by PCR and then subcloned into pET28a(+). The recombinant SjTnT protein (rSjTnT)was expressed in Escherichia coli BL21(DE3)cells. The serum specific to rSjTnT was prepared by immunized BALB/c mice with the recombinant antigen,and the immunogenicity of rSjTnT was detected by Western blotting and ELISA. The immuno-protective efficacy induced by rSjTnT in BALB/c mice was evaluated according to the reduction in worm and egg counts. Results The cDNA encoding SjTnT was successfully cloned and expressed in E. coli. Western blotting showed that rSjTnT had a good immunogenicity. The high level of specific IgG antibodies was detected,and 33.89% worm reduction and 43.94% liver egg reduction were obtained in mice vaccinated with rSjTnT combined with Seppic 206 adjuvant compared with those in the adjuvant control group. Conclusions rSjTnT could induce partial immuno-protection against S. japonicum infec-tion in BALB/c mice. This study provided a basic for understanding the biological function of SjTnT.
