The Change of Bone Metabolism in Ovariectomized Rats : Analyses of MicroCT Scan and Biochemical Markers of Bone Turnover.
10.3340/jkns.2012.51.6.323
- Author:
Kyung Hyuk YOON
1
;
Dae Chul CHO
;
Song Hee YU
;
Kyoung Tae KIM
;
Younghoon JEON
;
Joo Kyung SUNG
Author Information
1. Department of Neurosurgery, Daegu Veterans Hospital, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Bone loss;
Bone turnover;
Osteoporosis;
Ovariectomy;
Rat
- MeSH:
Alkaline Phosphatase;
Animals;
Biomarkers;
Bone Density;
Bone Resorption;
Collagen Type I;
Female;
Humans;
Lumbar Vertebrae;
Osteocalcin;
Osteogenesis;
Osteoporosis;
Ovariectomy;
Rats;
Rats, Sprague-Dawley;
Salicylamides;
X-Ray Microtomography
- From:Journal of Korean Neurosurgical Society
2012;51(6):323-327
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The purpose of this study was to verify the appropriateness of ovariectomized rats as the osteoporosis animal model. METHODS: Twelve female Sprague-Dawley rats underwent a sham operation (the sham group) or bilateral ovariectomy [the ovariectomy (OVX) group]. Eight weeks after operations, serum biochemical markers of bone turnover were analyzed; osteocalcin and alkaline phosphatase, which are sensitive biochemical markers of bone formation, and C-terminal telopeptide fragment of type I collagen C-terminus (CTX), which is a sensitive biochemical marker of bone resorption. Bone histomorphometric parameters and microarchitectural properties of 4th lumbar vertebrae were determined by micro-computed tomographic (CT) scan. RESULTS: The OVX group showed on average 75.4% higher osteocalcin and 72.5% higher CTX levels than the sham group, indicating increased bone turnover. Micro-CT analysis showed significantly lower bone mineral density (BMD) (p=0.005) and cortical BMD (p=0.021) in the OVX group. Furthermore, the OVX group was found to have a significantly lower trabecular bone volume fraction (p=0.002). CONCLUSION: Our results showed that bone turnover was significantly increased and bone mass was significantly decreased 8 weeks after ovariectomy in rats. Thus, we propose that the ovariectomized rat model be considered a reproducible and reliable model of osteoporosis.
