The expression of splice variant of progesterone-induced blocking factor in hepatocellular carcinoma
10.3969/j.issn.1006-5725.2014.10.020
- VernacularTitle:孕酮诱导阻断因子剪接体在肝细胞性肝癌中的表达
- Author:
Shuang WU
;
Shun LEI
;
Jie ZHOU
;
Rongbin ZHOU
;
Rui ZENG
;
Houjie LIANG
- Publication Type:Journal Article
- Keywords:
Progesterone-induced blocking factor;
Hepatocellular carcinoma;
Serum
- From:
The Journal of Practical Medicine
2014;(10):1581-1584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of splice variant of progesterone-induced blocking factor (PIBF) of healthy adult and hepatocellular carcinoma (HCC) patients. Methods Western blot was used to find out the expression in 3 normal livers and tumor and para-tumor tissue of 8 HCC patients. Use T Test to compare the difference. Immunohistochemistry was used to detect the expression in tumor and para-tumor tissue of 48 HCC patients. The splice variant of PIBF serum concentrations of 103 healthy adult, pre-operation and part of 5 days post-operation of 109 HCC patients was tested with ELISA. Result The expression of HCC tissue is lower than para-tumor tissue. The serum concentrations of healthy adult and HCC patients is 73.04 ± 6.29 ng/mL and 49.10 ± 4.07 ng/mL, and the difference was statistically significant (P=0.010). The analysis of blood concentrations of pre-operation and part of 5 days post-operation of 15 HCC patients indicate that the expression of pre-operation is lower than post-operation, and the difference was statistically significant (P = 0.008). Conclusion The splice variant of PIBF is low expressed in HCC tissue and serum. PIBF is a potential protein plays important role in cancer.
