The comparison among blood cell analyzer method, mononuclear cell direct counting and smear staining method in mononuclear cell count

VernacularTitle:血细胞分析仪法及直接计数法和涂片染色法单核细胞分类计数的比较
Author: Yulong ZHU ; Huiyu YU ; Zaorong LI
Publication Type:Journal Article
Keywords: Blood cells; Mononuclears; Direct counting; Manual microscope; Correlation
From: Chinese Journal of Postgraduates of Medicine 2014;37(15):32-34
CountryChina
Language:Chinese
Abstract: Objective To.investigate the accuracy and reliability of monocyte count of three methods.Methods The specimens classified by blood cell analyzer WBC monocytes ＜ 15％,15％-20％,＞ 20％ were taken randomly,draw 20 μ l after mixing,put in monocyte direct count dilution and count,and underwent routine smear,stained by Swiss-Gibbs and manual classified by microscope,and then compared the results of the three methods.Collected one copy each of the high,medium and low proportion of monocytes specimens,and counted 30 times by the three counting method.Results Blood cell analyzer in WBC monocytes 15％-20％,＞ 20％ had poor correlation with the results of the other two methods.Repeatability comparison of three methods,medium and low sample were better with method 1 and 2,high value sample was good with method 3.Conclusion The specimens of monocytes ≥ 15％,especially scatter plot is abnormal,must be confirmed by microscopic examination.