Study on the molecular size distribution and the structural characteristics of group B meningococcal cap-sular polysaccharides
10.3760/cma.j.issn.0254-5101.2014.05.013
- VernacularTitle:B 群脑膜炎球菌荚膜多糖分子大小分布和结构特性分析
- Author:
Zhiqiang ZHAO
;
Yingying YANG
;
Xubo YU
;
Yiyang FENG
;
Ani LI
;
Hongchun FANG
;
Ruijie QIAO
;
Bing WU
;
Fanglei LIU
;
Guilin XIE
- Publication Type:Journal Article
- Keywords:
Group B meningococcus;
Capsular polysaccharide;
KD;
Repeat units
- From:
Chinese Journal of Microbiology and Immunology
2014;(5):381-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular size distribution and the structure of group B me-ningococcal capsular polysaccharides for the development of vaccines .Methods The molecular size distribution of group B meningococcal capsular polysaccharides was analyzed by chromatography on a Sepharose CL -4B col-umn.The molecular weight of repeat units were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).The structural characteristics of group B meningococcal capsular polysaccharides were analyzed by nuclear magnetic resonance ( NMR) based on the chemical shift of all charac-teristic protons by using group C meningococcal capsular polysaccharides and sialic acid as the controls .Results The KD value of group B meningococcal capsular polysaccharides extracted from 15 strains were ranged from 0.60 to 0.76.The molecular weight of repeat units was 284, which was identical to the theoretical value .The group B meningococcal capsular polysaccharides were 2→8 linked homopolymers of sialic acid lacking O-acetyl groups.Conclusion The group B meningococcal capsular polysaccharides had lower molecular weights , which might result in their poor immunogenicity .The structure of group B meningococcal capsular polysaccharides could be quickly and accurately analyzed by NMR technology .
