Complete nucleotide sequence of a human echovirus 30 strain KM/A363/09 isolated in Yunnan, China in 2009
10.3760/cma.j.issn.0254-5101.2014.05.014
- VernacularTitle:2009年云南省 Echo30分离株 KM／A363／09全基因组序列分析
- Author:
Wei BAI
;
Junying CHEN
;
Yue PAN
;
Yanju ZHU
;
Congwen SHAO
;
Jiansheng LIU
;
Shaohui MA
- Publication Type:Journal Article
- Keywords:
Echovirus 30;
Complete genome;
Sequence analysis
- From:Chinese Journal of Microbiology and Immunology
2014;(5):388-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the genetic characteristics of the complete genome of a human echovirus 30 (Echo30) KM/A363/09 strain isolated in Yunnan, China in 2009.Methods Primers specif-ic for Echo30 were designed .The extracted RNA was amplified by using RT-PCR.Seven fragments covering the complete viral genome were sequenced and the complete sequences were aligned with other sequences of enterovirus reference strains downloaded from Genbank . By using Mega5.1, Geneious, RDP3 and SimPlot3.5.1 softwares, the phylogenetic and recombination analysis were carried out .Results The com-plete nucleotide sequence of KM/A363/09 isolate was 7425 bp in length, encoding 2194 amino acids.KM/A363/09 isolate was highly similar with Bastianni prototype strain showing the homology of 81.2%in nucle-otide and 95.8%in amino acid.The eight Echo30 isolates shared 81.2%-88.6%homologies in nucleotide sequences and 95.8%-97.8%in amino acid sequences .Phylogenetic analysis showed that the KM/A363/09 strain belonged to one clade of Echo 30 in China.The genetic recombination of KM/A363/09 isolate was detected in the non-structural region .Conclusion KM/A363/09 isolate belongs to one clade of Echo 30 in China indicating that the evolution of Echo 30 has occurred in China .
