Effects of alcohol on HCV replication and its mechanism
10.3760/cma.j.issn.0254-5101.2014.05.016
- VernacularTitle:酒精对丙型肝炎病毒复制的影响及机制研究
- Author:
Hui CHEN
;
Jinming SU
;
Yu LI
;
Rongfeng CHEN
;
Jie LIU
;
Minlian WANG
;
Fangning ZHAO
;
Hong WANG
;
Jinrong ZENG
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
Alcohol;
Type I interferon;
Innate immunity
- From:Chinese Journal of Microbiology and Immunology
2014;(5):397-401
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of alcohol on hepatitis C virus( HCV) replication and type I interferon signaling pathway in human hepatocytes .Methods Primary hepatocytes were treated with different concentrations of alcohol , and then infected with HCV .The infected cells were collected to measure the level of HCV RNA .The alcohol-treated hepatocytes were also collected to detect the expression of HCV Core, IFN-α, IFN-β, IRF-7, suppressor of cytokine signaling SOCS-2 and SOCS-3 at mRNA and protein levels by real-time quantitative PCR and ELISA or Western blot , respectively .Results Alcohol treatment enhanced HCV infection and replication in primary hepatocytes at concentrations higher than 10 mmol/L in a dose-dependent manner (P<0.05).Treatment with 40 mmol/L of alcohol significantly reduced the expression of IFN-α, IFN-βand IRF-7 at mRNA and protein levels , and increased the expression of SOCS-2 and SOCS-3 at mRNA and protein levels .Conclusion Alcohol treatment could damage the host in-nate immunity in human hepatocytes and promote HCV replication by reducing the expression of type Ⅰinter-feron ( IFN-αand IFN-β) and IRF-7 and increasing the expression of negative regulators including SOCS-2 and SOCS-3.These results demonstrated that the impairment of innate immunity in liver of alcohol abusers might contribute to the enhancement of HCV infection and result in poor therapeutic effect of IFN -α.
