JNK2 enhances the NKG2D-mediated natural killer cell cytotoxicity

VernacularTitle:JNK2可增强NKG2D介导的自然杀伤细胞肿瘤杀伤效应
Author: Xiaoqing ZHANG ; Yan SNU ; Xi MA ; Yang WANG ; Ingling LUL
Publication Type:Journal Article
Keywords: Tumor immunity; Cell killing; c-Jun N-terminal kinase; Natural killer cell; NKG2D
From:Chinese Journal of Microbiology and Immunology 2014;(5):331-336
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of c-Jun N-terminal kinase (JNK) 2 on NKG2D-mediated natural killer ( NK) cell cytotoxicity .Me thods NK cells were activated by polyinosinic-polycyti-dylic acid ( Poly I∶C ) .The activation status of JNK signaling pathway was detected .The cytotoxicity of ac-tivated NK cells and the level of IFN-γproduced were measured to determine the function of NK cells in the absence of JNK2.Tumor growth in wild type and JNK2-knockout (JNK2-/-) mice with lymphoma xenograft were measured to evaluate immune surveillance of NK cells .Results The phosphorylation of JNK and up-stream kinases were observed in the early stage of cell activation after treatment of Poly I ∶C.The expressed ligands of the activating receptors NKG2 D significantly increased NK cell cytotoxicity to lymphoma cells . JNK2 deficiency impaired the antitumor effects of NK cells , and then resulted in enhanced tumor growth in JNK2-/-mice.Conclusion JNK2 signaling pathway is involved in the NKG2 D-mediated activation of NK cells and regulates immune surveillance of NK cells against tumor .