Experiment study on screening efffective Mcl-1-targeted siRNA squence in gastric cancer cells
10.3969/j.issn.1006-5725.2014.09.006
- VernacularTitle:胃癌细胞Mcl-1基因有效siRNA序列筛选的实验研究
- Author:
Jinlu LIU
;
Zhen WANG
;
Junqiang CHEN
;
Xigang CUI
;
Pengfei MA
;
Bopei LI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
siRNA;
Mcl-1
- From:
The Journal of Practical Medicine
2014;(9):1359-1362
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of Mcl-1 gene in gastric cancer cell lines SGC7901 and MGC-803, and to screen the most effective Mcl-1-targeted siRNA sequence. Methods Mcl-1 expression was evaluated in human gastric cancer cell lines SGC7901 and MGC-803 by RT-PCR. Four segments of siRNAs (siRNA1, siRNA2, siRNA3 and siRNA4) targeting Mcl-1 mRNA and a no-sense control segment were designed by bioinformatic technology . Mcl-1 specific siRNAs were transfected transiently into SGC7901 and MGC-803 cells by using lipofectamine 2000 . After transfected 24 , 48 and 72 h , quantitative real-time PCR was applied to detect the mRNA expression of Mcl-1 and western-blot analysis was applied to detect the protein expression. Results Mcl-1 gene was expressed in both SGC7901 and MGC-803 cells. Overall, siRNA1 exhibited the best inhibitory effect after being transfected for 48h. The inhibition rate of mRNA level in SGC7901 group and MGC-803 group was 73.8%and 67.6%, and the inhibition rate of protein level in SGC7901 group and MGC-803 group was 79.3%and 96.1%. Conclusion Mcl-1 specific siRNA sequences were successfully designed, and siRNA1 was selected as the most effective sequence, which can simultanandeously inhibit the expression of Mcl-1 in GC7901 and MGC-803 cells.
