Atorvastatin down-regulating IL-1β expression in myocyte of aging rat by PPARβ/δ signal channel
10.3969/j.issn.1006-5725.2014.09.007
- VernacularTitle:阿托伐他汀通过PPARβ／δ途径下调老年大鼠心肌细胞IL-1β表达
- Author:
Lei HAN
;
Ping YE
;
Minggao LI
- Publication Type:Journal Article
- Keywords:
Aging;
Atorvastatin;
Peroxisome proliferator activated receptorβ/δ;
Interleukin-1β;
Rat;
Myocyte
- From:
The Journal of Practical Medicine
2014;(9):1363-1366
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between atorvastatin inhibiting Interleukin-1β(IL-1β) expression and peroxisome proliferator activated receptor β/δ (PPARβ/δ) signal channel in myocyte of aging rat. Methods Primary culture of myocyte were got from aging rat. Myocyte were divided into control group, DMSO group, atorvastatin group, atorvastatin plus GSK0660 group, whichwere treated respectively by Cell culture medium, dimethyl sulfoxide (DMSO), atorvastatin, atorvastin plus GSK0660. The expression level of IL-1βmRNA and protein was evaluated by RT-PCR and Western Blot respectively. Results (1)NO difference were found between control group and DMSO group in expression level of IL-1βmRNA and protein (P>0.05);(2)The expression level of IL-1βmRNA and protein in atorvastatin group were significantly lower than those of control group (P < 0.01); (3)Both mRNA and protein expression level of IL-1βin atorvastatin plus GSK0660 group were higher than those of atorvastatin group(P<0.05 or P<0.01), but still lower than those of control group(P<0.05). Conclusions Atorvastatin down-regulate the expression of IL-1βin aging myocytes by activating PPARβ/δsignal Channel.
