Inhibition of mitochondrial permeability transition pore opening reduced Aβ-mediated inflammatory effects on the apoptosis of neural stem cell
10.3969/j.issn.1006-5725.2014.09.012
- VernacularTitle:抑制线粒体通透性转换孔的开放削弱淀粉样β蛋白介导的炎症对神经干细胞的凋亡作用
- Author:
Qingzhuang CHEN
;
Ning ZHU
;
Jizhong LIN
;
Yan WAN
;
Kewan WANG
;
Yong WANG
- Publication Type:Journal Article
- Keywords:
Amyloid beta-protein;
Mitochondrial permeability transition pore;
Microglia;
Neural stem cells;
Inflammatory response
- From:
The Journal of Practical Medicine
2014;(9):1380-1384
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study is to investigate the protective effect of inhibiting the open of mitochondrial permeability transition pore on neural stem cells. Methods In present study, four groups were set up, such as control, conditioned medium control group, Aβ1-42 group and CsA group. The levels of inflammatory mediators were detected by LiquiChip technique. The apoptotic rate of neural stem cells was detected by flow cytometry and the expression of caspase-3 was confirmed by western blotting. Results The levels of IL-6 and TNF-αwere 7.92 and 1.22 times higher than those in control group after Aβ1-42 acting on microglia for 96 h. After being exposed to inflammatory media, the apoptotic rate of neural stem cells reached 41.17%, these was significant increase compared to control (P<0.001);while the apoptotic rate were decrease significantly if the open of the mitochondrial permeability transition pore was inhibited. In the meantime, the activation of caspase-3 was reduced obviously. Conclution Inhibiting the open of mitochondrial permeability transition pore can markedly reduced the apoptotic rate of neural stem cells , and dramatically impaired the effect of inflammatory on the apoptosis of neural stem cells , suggesting that inhibiting the opening of the mitochondrial permeability transition pore have protective effect on neural stem cells.
