The influence of B、T lymphocytes decreased in Apo E gene knockout mice
10.3969.j.issn.1671.7856.2014.005.002
- VernacularTitle:ApoE 基因敲除对小鼠 B、T 淋巴细胞的影响
- Author:
Guiying SHI
;
Haitao ZHANG
;
Lianfeng ZHANG
;
Lin BAI
- Publication Type:Journal Article
- Keywords:
Apo E gene knockout mice;
Flow cytometry;
Hematopoietic stem cell
- From:
Chinese Journal of Comparative Medicine
2014;(5):5-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of Apo E gene knockout on the lymphocytes . Methods Cells from the peripheral blood, spleen and bone marrow of Apo E knockout and wildtype mice were stained with kinds of antibodies , and analyzed by flow cytometry .Results Compared with wildtype mice , significant differences were found in B and T lymphoctes in the peripheral blood and spleen , but there was no significant difference in pre B cells , T lymphocytes in the thymus and long term hematopoietic stem cell in the Apo E knockout mice .Conclusion Numbers of B lymphocytes decreased in the peripheral blood and spleen , but there was no significant difference in B , T lymphocytes development , and numbers of long term hematopoietic stem cell in Apo E gene knockout mice .
