The study on organ weights, blood physiological and biochemical parameters and immune cells in Rag2 knockout mice
10.3969.j.issn.1671.7856.2014.005.012
- VernacularTitle:Rag2敲除小鼠脏器重量、血液生理生化指标及免疫细胞的研究
- Author:
Yu WANG
;
Xuying WU
;
Zhaosheng WEI
;
Jinfeng WU
;
Huizhen LIU
;
Yushu TIAN
;
Wenming ZHANG
;
Zhiwei YANG
- Publication Type:Journal Article
- Keywords:
Rag2 knockout mice;
Organ weights;
Blood physiological and biochemical parameters;
Immune cells
- From:
Chinese Journal of Comparative Medicine
2014;(5):56-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the organ weights , blood physiological and biochemical parameters , and immune function of Specific Pathogen Free ( SPF) Rag2 knockout ( KO) mice.Methods Rag2 knockout mice were selected at five and ten weeks , and the organ weights , blood physiological and biochemical parameters were observed .The percentages of CD3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were checked by FCM in Rag2 KO mice at six week of age in terms of its T , B lymphocyte function and NK cell activity .Results Among the same sex group of NOD/SCID mice, the weights of brain, lung, spleen, liver, heart, kidney, and the levels of TBIL, WBC in 10 weeks of age are higher than 5 weeks.At the same age, the weights of heart, kidney, liver, spleen, and the levels of AST, ALP, A/G, GLU, PLT, PCT, WBC, and LYM%in male mice are higher than females.The Rag2 KO mice lacks T cells(0.36 ±0.15)%、CD4+T cells(0.21 ±0.06)%、CD8+T cells(0.23 ±0.07)%、CD19+B cells(0.28 ±0.04)%、B220+B cells(2.03 ±0.42)%).The percentage of NK cells is(24.13 ±3.62)%, and the percentage of granulocytes is (57.20 ±3.85)%.Conclusion The study suggests that the organ weights , blood physiological and biochemical parameters are affected by age and gender in Rag2 KO mice, which main biological characteristics are similar with C 57BL/6J mice.The Rag2 KO mice show the deficiency of T , B cells function .
