Gene silencing of 90K/Mac-2BP enhances the apoptosis of U937 cells by HIV-1 infection
10.3969.j.issn.1671.7856.2014.005.003
- VernacularTitle:90 K／Mac-2 BP 基因沉默增强 HIV-1感染的单核巨噬细胞凋亡
- Author:
Chunyan FU
;
Hong JIANG
;
Jing XUE
;
Zhe CONG
;
Ting CHEN
;
Qiang WEI
- Publication Type:Journal Article
- Keywords:
U937;
90K/Mac-2BP;
HIV-1;
Apoptosis
- From:
Chinese Journal of Comparative Medicine
2014;(5):10-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cell death by HIV-1 infection on gene 90K/Mac-2BP by RNA interference (RNAi) in U937 cell line.Methods We used human monocyte-macrophage cell line U937 as the cell model.Cells were infected by HIV-1 ( R5-tropic) 5 days, and then stained by PE-Annexin V and PerCP-7-AAD.90K/Mac-2BP in U937 cell line was knocked down , and these cells were infected by HIV-1 for 5 days.Then, cells were stained by PE-AnnexinV and PerCP-7-AAD.Apoptosis were examined upon flow cytometry .Results The percentages of Annexin V+cells without 90K/Mac-2BP knock-down were (16.27 ±0.30)% by HIV-1 infection.The percentages of them with 90K/Mac-2BP knock-down were (31.26 ±0.35)%, (25.76 ±0.30)%, (23.69 ±0.33)% respectively.The increase of cell apoptosis rate for HIV-1-infected U937 cells by 90K/Mac-2BP siRNA transfection was significantly greater than that for HIV-1-infected untreated cells (P﹤0.01).Conclusion The apoptosis of HIV-1-infected U937 cells was regulated by the expression of 90K/Mac-2BP.
