The study on organ weights, blood physiological and biochemical parameters and immune cells in NOD/SCID mice
10.3969.j.issn.1671.7856.2014.005.010
- VernacularTitle:NOD／SCID 小鼠主要脏器重量、血生理生化指标和免疫细胞的测定
- Author:
Huaiyuan WANG
;
Xuying WU
;
Wenming ZHANG
;
Yushu TIAN
;
Jinfeng WU
;
Yu WANG
;
Yajing ZHANG
;
Zhiwei YANG
- Publication Type:Journal Article
- Keywords:
NOD/SCID mice;
Organ weights;
Blood physiological and biochemical parameters;
Immune cells
- From:
Chinese Journal of Comparative Medicine
2014;(5):47-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the organ weights , physiological and biochemical parameters and immune cells of NOD/SCID mice.Methods Mice at five and ten weeks of age were selected .The organ weights, blood physiological and biochemical parameters were observed .The percentages of CD 3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were checked by FCM in NOD/SCID mice at six week of age in terms of its T , B lymphocyte function and NK cell activity.Results Among the same sex group of NOD/SCID mice, the weights of kidney, liver, heart and lung, and the blood physiological indexes of RBC , HGB, HCT, MCV, MCH, RDW, and the blood biochemical indexes of TP , ALB, ALP, CHO, TBIL show significant difference between 5 and 10 weeks.At the same age, HCT, GLOB, A/G, CHO, TG, TBIL and the UN are significantly different between male and female NOD /SCID mice.NOD/SCID mice lack T cells (0.37 ±0.26)%、CD4+T cells (0.35 ±0.13)%、CD8 +T cells (0.47 ±0.10)%、CD19+B cells (0.13 ± 0.05)%、B220+B cells (1.20 ±0.44)%.The percentage of NK cells is (6.90 ±0.82)%, and the percentage of granulocytes is (47.88 ±15.54)%.Conclusions The study indicates that NOD/SCID mice show the deficiency of T , B and NK cells function .The organ weights , blood physiological and biochemical parameters are affected by age and gender . The studied parameters of NOD/SCID mice are similar with the same strain in other countries .
