PTPMeg2 inhibits STAT3 transcriptional activity in NIH3 T3/STAT3 CA cell model
10.3969/j.issn.1001-1978.2014.06.023
- VernacularTitle:PTPMeg2抑制NIH3 T3/STAT3 CA细胞模型STAT3转录活性的研究
- Author:
Fuqin SU
;
Yuchun WANG
;
Zhanpeng QI
;
Chao SUN
;
Jincai HOU
- Publication Type:Journal Article
- Keywords:
PTPMeg2;
NIH3 T3/STAT3 CA;
cell model;
constitutively activated STAT3;
proliferation;
transcriptional activity
- From:
Chinese Pharmacological Bulletin
2014;(6):843-847
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the NIH3T3/STAT3CA cell proliferation ability and the STAT3 transcriptional activity affected by PTPMeg2 . Methods MTT assay and xenograft nude mice model were used to investigate the NIH3 T3/STAT3 CA cell proliferation inhibited by PTPMeg2 in vitro and in vivo. Co-immunoprecipitation assay was used to measure the interaction between PT-PMeg2 and STAT3CA. STAT3 transcriptional activity was measured by dual-luciferase assay. Results The NIH3 T3/STAT3 CA cell proliferation ability was signifi-cantly inhibited by PTPMeg2 in vitro and in vivo com-pared with the control group ( P <0.05 ) . The tran-scriptional activity was increased by PTPMeg2 , but not the PTPMeg2 mutant (PTPMeg2C515S) and the ShPT-PMeg2 . Conclusion PTPMeg2 plays a role in inhibi-ting the proliferation ability of NIH3 T3/STAT3 CA cells through inhibiting the STAT3 transcriptional activity.
