Effect of resveratrol on proliferation and differentiation in K562 cells
10.3969/j.issn.1001-1978.2014.06.025
- VernacularTitle:白藜芦醇对K562白血病细胞抑制增殖和诱导分化的作用
- Author:
Yanna ZHAO
;
Ruilan GAO
;
Lipei WANG
;
Xiaoling YU
;
Liming YIN
- Publication Type:Journal Article
- Keywords:
resveratrol;
leukemia;
differentiation;
CD11b;
CD14;
CD42b;
GATA-1;
PU. 1
- From:
Chinese Pharmacological Bulletin
2014;(6):853-856
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of resveratrol on proliferation and differentiation in K562 cells. Methods K562 cells were treated with different con-centrations of resveratrol for 6d. The colony number of K562 cells was detected with semi-solid culture assay. Expression of GATA-1 and PU. 1 in K562 cells was re-spectively measured with immunocytochemistry and Western blot. Expression of differentiation related anti-gen, CD11b, CD14 and CD42b, was measured with flowcytometry on K562 cells. Results Resveratrol
could significantly decrease the colony number of K562 cells in a dose-dependent manner, and enhance the ex-pression of GATA-1,PU. 1,CD11b, CD14 and CD42b in K562 cells. Conclusion Resveratrol could inhibit the proliferation and induce differentiation of K562 cells via up-regulating the expression of GATA-1 and PU. 1 protein.
