Yes-associated protein modulation of human glioma cell growth invitro
10.3969/j.issn.1000-8179.20140237
- VernacularTitle:YAP调控人胶质瘤细胞生长的体外研究
- Author:
Fuhua YU
;
Zhifan JIA
;
Peiyu PU
;
Guangxiu WANG
;
Anling ZHANG
;
Weidong YANG
- Publication Type:Journal Article
- Keywords:
glioma;
YAP;
Hippo signal pathway;
apoptosis;
invasion
- From:
Chinese Journal of Clinical Oncology
2014;45(11):689-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to explore the effect of Yes-associated protein (YAP) on the growth of the human glioma cell line LN229. Methods:YAPsiRNA was transfected into LN229 cells to knock down the YAP expression. The downregulation of the YAP expression was identified through Western Blot analysis. Colorimetric assay using methyl-thiazolyl-tetrazolium was applied to evaluate cell proliferation ability. Cell invasive activity was examined using Transwell assay. Flow cytometry and AnnexinV were used to detect cell cycle and apoptosis, respectively. The relevant molecules regulating proliferation, invasion, cell cycle progression, and apoptosis were examined through Western Blot analysis. Results:The YAP expression was downregulated after YAPsiRNA was trans-fected into LN229 glioma cells. Reduced YAP expression could arrest the cell cycle at G0/G1 phase, inhibit cell proliferation and inva-sion, and promote apoptosis. The expression of the proliferating cell nuclear antigen (Ki-67), matrix metallopeptidase-9 (MMP-9), cy-clin D1, and Bcl-2 were downregulated. Conclusion:The downregulation of YAP in LN229 cells suppresses cell proliferation and inva-sion, as well as promotes cell apoptosis. This study provides a novel evidence for further study on Hippo-YAP signal pathway in molec-ular pathology of glioma.
