Effects of trichosanthin on apoptosis and cytoskeleton microtubule structure reconfiguration in lung cancer A549 cells
10.3969/j.issn.1000-8179.20140473
- VernacularTitle:天花粉蛋白对肺癌A549细胞凋亡及细胞骨架微管结构改变的研究
- Author:
Jing ZHUANG
;
Changgang SUN
;
Congcong WANG
;
Fubin FENG
;
Qingliang LV
;
Huaqing WANG
;
Mianhua WU
- Publication Type:Journal Article
- Keywords:
trichosanthin;
lung cancer;
apoptosis;
cytoskeleton
- From:
Chinese Journal of Clinical Oncology
2014;45(11):693-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to explore the effects of trichosanthin on the proliferative inhibition and apoptosis induc-tion in human lung carcinoma A549 cells. Methods:A549 cells were treated with various concentrations of TCS. The inhibitory effects in proliferation were detected by the MTT method. The microfilament changes were observed by transmission electron microscopy. Apoptosis rate and cell cycle were determined by flow cytometry. Results:A549 cells treated with TCS presented apoptotic changes and decreased cell activity. When the concentration increased and time was prolonged, the inhibition rate increased correspondingly. Conclusion:Pharmacological doses of TCS inhibited the proliferation and differentiation in lung carcinoma A549 cells and affected the function in A549 cells by changes in the cytoskeleton.
