Study on Quality Control of New Medicinal Resources of Pineapple Leave
10.11842/wst.2014.05.005
- VernacularTitle:新药用资源菠萝叶的质量标准研究
- Author:
Jingfei JIANG
;
Wei WANG
;
Zhenguo LI
;
Fan LEI
;
Weidong XIE
;
Yi DING
;
Dongming XING
;
Lijun DU
- Publication Type:Journal Article
- Keywords:
Pineapple leave;
quality control;
ananasate;
p-coumaric acid
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(5):972-977
- CountryChina
- Language:Chinese
-
Abstract:
This article was aimed to study the quality control of pineapple leaf in order to further develop the appli-cations of pineapple leave as medicinal resources. The tissue structure of pineapple leaf was observed by tissue biop-sy assay. Thin-layer chromatography (TLC) was employed on the qualitative identification of ananasate in the leave. The p-coumaric acid and ananasate were detected with high-performance liquid chromatography (HPLC) assay in the content determination. The results showed that pineapple leaf can be divided into the upper and lower leaf epidermis with stoma under microscope observation. Ananasate of the leave can be indentified clearly by TLC. HPLC method can accurately detect the content of p-coumaric acid and ananasate of pineapple leave. There are different contents of p-coumaric acid and ananasate of pineapple leave in different origins. In Y unnan province, the contents of p-coumaric acid and ananasate of pineapple leave are the highest; while the contents of p-coumaric acid and ananasate in Guangxi province are the lowest. It was concluded that ananasate can be used in the quality identification. The p-coumaric acid and ananasate can be used in the quantitative determination in the better control of the quality of pineapple leaf.
