Effect of Cerebrospinal Fluid with Drug-containing Modified San-Jia-San Decoction on IL-1α, IL-1βand IL-6 of Hippocampal Neurons Model Injury Induced by Aβ25-35
10.11842/wst.2014.05.010
- VernacularTitle:“改良三甲散”含药脑脊液对Aβ25-35诱导海马神经神经元细胞损伤IL-1α、IL-1β和IL-6的影响
- Author:
Jinjuan WU
;
Geng ZHANG
;
Miao JIANG
;
Tao LIU
- Publication Type:Journal Article
- Keywords:
Modified San-Jia-San decoction;
cerebrospinal fluid;
hippocampal neuron;
IL-1α;
IL-1β;
IL-6
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(5):1005-1009
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate effect on the expression of cell inflammatory factor IL-1α, IL-1β and IL-6 of modified San-Jia-San (SJS) decoction on cultured hippocampal neurons injury induced by β-amyloid 25-35 protein (Aβ25-35). Primary cultured hippocampal neurons were divided into the normal group, model group, huperzine A group, low-dose SJS group, middle-dose SJS group, and high-dose SJS group. After selective culture for 7~10 days and the absorption of culture fluid, the blank culture fluid, normal cerebrospinal fluid (normal saline group), huperzine A cerebrospinal fluid, and low-, middle- and high-dose SJS cerebrospinal fluid were added, respectively. The culture fluid was added up to an equivalent medium. The incubation was 2 h under the temperature of 37℃. Ex-cept the normal group, Aβ25-35 dealt with aging (the final concentration was 5 μmol/L) was added to other groups to establish AD cell model. An equivalent amount of culture fluid was added to the normal group. After 24 h of incuba-tion, the cell culture supernatant was collected. Enzyme-linked immunosorbent assay (ELISA) was used in the content detection of IL-1α, IL-1β and IL-6. The results showed that modified SJS decoction can decrease the content of IL-1α, IL-1β and IL-6 in the cell culture supernatant. It was concluded that the modified SJS decoction can effectively inhibit the expression of cell inflammatory factor IL-1α, IL-1β and IL-6. It had certain anti-inflammatory effect to protect hippocampal neurons.
