Effects of Bai-Zhu Fu-Ling Decoction in Different Proportioning on VIP and Its Receptor in Crohn's Disease Rats with Spleen Deficiency Syndrome
10.11842/wst.2014.05.028
- VernacularTitle:白术茯苓汤不同配比对脾虚型克罗恩病大鼠结肠VIP及其受体的影响
- Author:
Yueqiang WEN
;
Bo JIA
;
Xinglong LIU
;
Jie ZHU
;
Fenghua ZHANG
;
Cen JIANG
;
Teng PENG
;
Xiaohong LI
- Publication Type:Journal Article
- Keywords:
Bai-Zhu Fu-Ling Decoction;
proportioning;
Crohn's disease with spleen deficiency syndrome;
VIP;
receptor
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(5):1122-1127
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the effect of Bai-Zhu Fu-Ling (BZFL) Decoction in different proportion-ing on VIP and VIPR1 in Crohn's disease (CD) rats with spleen deficiency syndrome, in order to further explore the immunologic mechanism of BZFL Decoction on CD. The CD rat model with spleen deficiency syndrome was estab-lished using exhaustion and hunger. The model rats were treated by BZFL Decoction with different proportioning, and immunohistochemistry (IHC) was used to detect the expression of VIP and its receptor in colon tissues. The results showed that comparing to the blank control group, the level of VIP and its receptor of the model group significantly increased (P< 0.05). Comparing to the model group, the level of VIP and its receptor in BZFL Decoction B5 group (Rhizoma A tractylodis Macrocephalae:Poria = 12:15), B6 group (Rhizoma A tractylodis Macrocephalae:Poria = 15:12) and B7 group (Rhizoma A tractylodis Macrocephalae:Poria = 18:9) was significantly decreased (P< 0.05). It was con-cluded that the effect of BZFL Decoction of B5 group, B6 group and B7 group was better than other groups in VIP and its receptor which can regulate the VIP and its receptor, inhibit the releasing of inflammatory factors and reduce intestinal inflammation injury.
