Inorganic sulfur reduces the motility and invasion of MDA-MB-231 human breast cancer cells.
- Author:
Jin Joo KIM
1
;
Ae Wha HA
;
Hee Sun KIM
;
Woo Kyoung KIM
Author Information
- Publication Type:Original Article
- Keywords: Inorganic sulfur; invasion; motility; matrix metalloprotease; MDA-MB-231 cells
- MeSH: Breast; Breast Neoplasms; Cell Movement; Collagen; Drug Combinations; Gene Expression; Humans; Laminin; Metalloproteases; Neoplasm Metastasis; Proteoglycans; RNA, Messenger; Sulfur
- From:Nutrition Research and Practice 2011;5(5):375-380
- CountryRepublic of Korea
- Language:English
- Abstract: This study investigated the effects of inorganic sulfur on metastasis in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured in the absence or presence of various concentrations (12.5, 25, or 50 micromol/L) of inorganic sulfur. Cell motility, invasion, and the activity and mRNA expression of matrix metalloproteases (MMPs) were examined. Numbers of viable MDA-MB-231 cells did not differ by inorganic sulfur treatment from 0 to 50 micromol/L within 48 h. Inorganic sulfur significantly decreased cell motility and invasion in the MDA-MB-231 cells in a dose-dependent manner (P < 0.05), as determined using a Boyden chamber assay and a Matrigel chamber. The activities of MMP-2 and MMP-9 were significantly reduced by inorganic sulfur in a dose-dependent manner (P < 0.05). The inorganic sulfur also significantly inhibited MMP-2 and MMP-9 expression in the cells (P < 0.05). These data suggest that inorganic sulfur can suppress cancer cell motility and invasion by inhibiting MMP-2 and MMP-9 activity and gene expression in MDA-MB-231 cells.
