Effect of sevoflurane anaesthesia on expression of N-methyl-D-aspartate receptor in developing hippocampal neurons in neonatal rats
10.3760/cma.j.issn.0254-1416.2014.03.005
- VernacularTitle:七氟醚麻醉对新生大鼠远期海马神经元NM DA 受体表达的影响
- Author:
Xiaohong TANG
;
Yize LI
;
Chunyan WANG
;
Meihua YANG
;
Miaomiao WANG
;
Guolin WANG
- Publication Type:Journal Article
- Keywords:
Anesthetics,inhalation;
Infant,newborn;
Receptors,N-methyl-D-aspartate;
Child development;
Hippocampus;
Neurons
- From:
Chinese Journal of Anesthesiology
2014;(3):275-278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sevoflurane anaesthesiaon the expression of N-methyl-D-aspartate (NMDA ) receptor in the developing hippocampal neurons in neonatal rats .Methods Sixty-four healthy male Sprague-Dawley rats ,aged 7 days ,weighing 10-15 g ,were randomly divided into 2 groups with 32 rats in each group using a random number table:control group (group C ) and sevoflurane anaesthesia group (group S ) . Animals in group C inhaled 30% oxygen for 6 h ,while animals in group S inhaled 3% sevoflurane for 6 h .Y-maze test was performed in the rats at 21 and 28 days after birth to evaluate the memory function .On 7 days after birth (immediately after the end of oxygen inhalation or sevoflurane anesthesia ) ,and 14 ,21 and 28 days after birth ,the expression of 1 ,2A and 2B subunits-containing NMDA receptors in the total protein and membrane protein in hippocampal neurons was determined by Western blot .The ratio of NMDA receptors in the membrane protein to those in the total protein (m/t ratio ) was calculated .Results Compared with group C ,the percentage of spontaneous alternation was significantly decreased on 21 and 28 days after birth ,the expression of 1 ,2A and 2B subunits-containing NMDA receptors in the membrane protein was down-regulated on 7-28 days after birth ,and m/t ratio was decreased in group S ( P<0.05) .There was no significant difference in the number of entries into each arm and expression of 1 ,2A and 2B subunits-containing NMDA receptors in the total protein between group C and group S ( P>0.05 ) .Conclusion The mechaism by which sevoflurane anaesthesia induces memory impairment in neonatal rats is related to inhibition of trafficking of NMDA receptors in the developing hippocampal neurons to the cell membrane ,and down-regulation of the number of NMDA receptors in the membrane protein .
