Simultaneous Determination of Naringin, Hesperidin and Atractylodin in Yun-Pi Powder Extract by HPLC

VernacularTitle:HPLC法同时测定运脾散浸膏中柚皮苷、橙皮苷和苍术素的含量
Author: Daye CHEN ; Wen LIU ; Xiaowei SHI ; Yu PENG
Publication Type:Journal Article
Keywords: Y un-Pi Powder extract; naringin; hesperidin; atractylodin; HPLC
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(5):1020-1024
CountryChina
Language:Chinese
Abstract: This study was aimed to simultaneously determinate the content of naringin, hesperidin and atractylodin in Y un-Pi (YP) Powder extract by HPLC. A Thermo ODS-2 column (250 mm í 4.6 mm, 5 μm) was used for the gra-dient elution with a methanol-water of 0~5 min (30%~40% A), 5~15 min (40%~50% A), 15~20 min (50%~79%A), 20~30 min (79%~100% A), 30~45 min (100% A), 45~55 min (100%~30% A), 55~60 min (30% A). The col-umn temperature was at 30℃. The detection wavelength was at 283 nm. The flow rate was 1 mL·min-1. The linear range was determined to be 0.009 16~0.137 00 μg (r = 0.999 9), 0.006 22~0.091 30 μg (r = 0.999 8), 0.003 58~0.053 70 μg (r = 0.999 9), respectively for naringin, hesperidin and atractylodin. The average recovery rate of pterodontic acid was 103.51%, 96.82% and 97.65%. And RSD was 1.37%, 1.41%, 1.49% (n = 6). It was concluded that the method had good specificity and repeatability. It was considered as a good tool for the content determination of naringin, hesperidin and atractylodin in YP Powder extract.