Effect of isoflurane preconditioning on inflammatory responses during spinal cord injury in rats
10.3760/cma.j.issn.0254-1416.2014.03.020
- VernacularTitle:异氟醚预处理对大鼠脊髓损伤时炎性反应的影响
- Author:
Lijuan ZHU
;
Lianhua ZHANG
;
Miaomiao LYU
;
Meiyan SUN
;
Changjun GAO
;
Hui ZHAO
;
Xude SUN
;
Lixian XU
- Publication Type:Journal Article
- Keywords:
Isoflurane;
Spinal cord injuries;
Systemic inflammatory response syndrome;
Ischemic preconditioning
- From:
Chinese Journal of Anesthesiology
2014;(3):328-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of isoflurane preconditioning on inflammatory responses during spinal cord injury (SCI ) in rats .Methods Sixty adult male Sprague-Dawley rats ,weighing 250-300 g , were randomly divided into 3 groups ( n= 20 each ) using a random number table :sham operation group (S group) , SCI group , and isoflurane preconditioning group (I group ) . The animals were anesthetized with intraperitoneal pentobarbital sodium 40 mg/kg .SCI was produced by a weight-drop contusion at the T10 level .The rats inhaled 2% isoflurane for 2 h ,and the model was established at 24 h after the end of isoflurane inhalation in I group . Neurological function was assessed and scored by using the the Basso , Beattie , Bresnahan (BBB ) Locomotor Rating Scale on 7 days after SCI .Five rats in each group were then chosen and spinal cord specimens were obtained and cut into sections which were stained with haematoxylin and eosin for determination of the viable neuron count .Fifteen rats in each group were sacrificed and the spinal cord was removed for detection of nuclear factor kappaB (NF-κB ) and interleukin-1β (IL-1β) expression (by Western blot ) .Results Compared with S group ,BBB score and the number of viable neurons were significantly decreased ,and the expression of NF-κB and IL-1βprotein was up-regulated in SCI group ( P<0.05) .Compared with SCI group ,BBB score and the number of viable neurons were significantly increased ,and the expression of NF-κB and IL-1βprotein was down-regulated in group I ( P<0.05 ) .Conclusion The mechanism by which isoflurane preconditioning protects the spinal cord is related to inhibition of inflammatory responses in rats .
