Relationship between Nrf2-ARE pathway and acute lung injury induced by endotoxic shock in rabbits
10.3760/cma.j.issn.0254-1416.2014.03.022
- VernacularTitle:Nrf2-A RE 通路与内毒素休克诱发兔急性肺损伤的关系
- Author:
Dan ZHENG
;
Jianbo YU
;
Lirong GONG
;
Man WANG
;
Yan XU
;
Yuan ZHANG
;
Li LI
;
Xinshun CAO
;
Daquan LIU
- Publication Type:Journal Article
- Keywords:
NF-E2-related factor 2;
Response elements;
Shock,septic;
Respiratory distress syndrome,adult
- From:
Chinese Journal of Anesthesiology
2014;(3):334-337
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the relationship between erythroid 2-related factor (Nrf2 )-antioxidant response element (ARE) pathway and acute lung injury (ALI) induced by endotoxic shock in rabbits .Methods Thirty healthy male New Zealand white rabbits ,aged 2 months ,weighing 1.5-2.0 kg ,were randomly divided into 3 groups (n=10 each) using a random number table :control group (group C) ,group ALI and all-trans retinoic acid group (group ATRA ) .In group ATRA ,all-trans retinoic acid 6 mg/kg (in filter sterilized vegetable oil 1.2 ml) was injected intraperitoneally once a day for 2 days .ALI was induced by lipopolysaccharide 5 mg/kg (in normal saline 2 ml ) injected via the auricular vein at 10 h after the last injection of ATRA in ALI and ATRA groups .The equal volume of normal saline was injected instead in group C .The rabbits were sacrificed at 6 h after lipopolysaccharide or normal saline administration .The pulmonary specimens were removed for determination of wet/dry lung weight ratio (W/D ratio ) and expression of Nrf2 mRNA and nuclear protein ,and HO-1 mRNA and protein in lung tissues .The pathological changes of lungs were scored .Results Compared with group C ,the pathological score and W/D ratio were significantly increased , and the expression of Nrf2 mRNA and nuclear protein ,and HO-1 mRNA and protein was up-regulated in ALI and ATRA groups ( P<0.05 ) .Compared with group ALI ,the pathological score and W/D ratio were significantly increased ,the expression of HO-1 mRNA and protein was down-regulated ( P<0.05) ,and no significant change was found in the expression of Nrf2 mRNA and nuclear protein in group ATRA ( P> 0.05 ) .Conclusion Activation of Nrf2-ARE pathway is the regulatory mechanism of the body adapting to the ALI induced by endotoxic shock in rabbits .
