Effect of Miao Nationality Herbs Spray for Serum SOD, MDA and Expression of Fas-mRNA and Caspase-3 mRNA in Lung Tissues of Silica-treated Rats
10.11842/wst.2014.05.037
- VernacularTitle:苗药组方雾化剂对染矽尘大鼠血清SOD、MDA和肺组织细胞凋亡Fas、Caspase-3基因表达的影响
- Author:
Gao HUANG
;
Guangzhi HE
;
Guixi SONG
;
Xia LIU
;
Feng CAO
;
Wenjia WANG
;
Yuchen LIU
;
Changfu YANG
;
Kun CAI
;
Bangxiang XU
;
Chuan QIN
;
Jinhuo WAN
;
Zhenlian LUO
;
Jianmei HUANG
- Publication Type:Journal Article
- Keywords:
Silica dust suspension;
Miao nationality herbs spray;
SOD;
MDA;
Fas gene
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(5):1172-1177
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to prepare the spraying agent of prescriptions of Miao nationality herb and investigate the effect of Miao nationality herbs spray for serum SOD, MDA, and expression of Fas and Caspase-3 mRNA in lung tissues of silica-treated rats. The healthy SD rats were divided into 5 groups. Silica dust suspension was used in the model establishment of 4 groups. After the model was successfully established, 3 groups were randomly selected and given glucocorticoids atomization inhalation, Miao nationality herbs spray, Miao nationality herbs spray combined with intragastric administration of herbal medicine, respectively. After 40-day treatment, water-solubletetrazolium salt (WST-1) was used in the detection of serum superoxide dismutase (SOD). Thiobarbituric acid (TBA) was used in the detection of malondialdehyde (MDA). The mRNA expression variance of the Fas and Caspase-3 were detected by RT-PCR. The results showed that compared with the silica dust suspension group, the SOD activity of serum in the Miao nationality herbs spray group was significantly increased (P< 0.05). MDA content and the mRNA of Fas and Caspase-3 were significantly lower in the Miao nationality herbs spray group (P< 0.05). It was concluded that Miao nationality herbs spray group was able to increase the SOD activity of serum, decrease MDA content, and obviously decrease the expression of Fas and Caspase-3 of lung tissues among silica dust suspension rats.
