Development and evaluation of candidate reference method for the determination of lithium in serum based on ion chromatography
10.3969/j.issn.1673-4130.2014.11.040
- VernacularTitle:离子色谱法测定血清锂离子候选参考方法的建立及性能评估
- Author:
Ji SUN
;
Min SHEN
;
Minmin TU
;
Lishan WU
- Publication Type:Journal Article
- Keywords:
serum lithium;
ion chromatography;
candidate reference method;
performance evaluation
- From:
International Journal of Laboratory Medicine
2014;(11):1468-1469
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a candidate reference method for the determination of serum lithium based on ion chromatog-raphy and evaluate its analytical performance.Methods A simple sample treatment procedure,which can be remove the proteins and/or organics in human serum,has been developed for the determination of serum lithium.Method precision was evaluated with different concentration of fresh human serum and EQA sample RELA-A/B.Method accuracy was investigated with the recovery ex-periments in fresh human serum and RELA-A/B sample.Results The linear equation was Y =0.817 1X -0.001 3 with a correla-tion coefficient of 0.999 95 under the optimum experimental conditions,the detection limit (3S/N)for lithium was 6 μg/L and the imprecision was less than 1.0%.The results of the recovery experiments indicated that the recoveries were reasonable for the deter-mination of serum lithium,in a range of 99%-101%.Conclusion The candidate reference method of lithium was successfully es-tablished and which can be used for traceability and standardization.It may provide an effective way for routine testing of lithium traceable to the reference method/reference material.
