Study on fluorescent quantum dot immunofiltration assay for quantitative detection of C-reactive protein
10.3969/j.issn.1673-4130.2014.11.042
- VernacularTitle:量子点荧光免疫渗滤法定量检测血清C反应蛋白的研究
- Author:
Weihua WU
;
Jia CHEN
;
Pengfei ZHANG
;
Guiping RAN
- Publication Type:Journal Article
- Keywords:
quantum dots;
fluorescence;
immunofiltration;
C-reactive protein
- From:
International Journal of Laboratory Medicine
2014;(11):1471-1473
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the feasibility of using fluorescent immunofiltration test based on quantum dots (QDs)for rapid and quantitative detection of C-reactive protein.Methods Based on homemade QDs and QDs-antibody bioconjugates,an immune de-tection method was established via the double antibodies sandwich technique on the immunochromatography card.The test results could be read under the irradiation of UV light,and quantitative results could be measured through the combination of a laser and fluorescent spectroscopy.Results Under UV light irradiation,the minimum detection concentration of CRP was 0.156 mg/L.U-sing the quantitative detection method,the fluorescent intensities on the cards could be established a linear relationship with the con-centration of CRP,and the linear equation was that log(Y )=0.563 log(X)+4.570,r2 =0.958.Conclusion The fluorescent quan-tum dot immunofiltration assay can be used for quantitative detection of CRP;The quantum dots immuno-labels have the potential to develop new type of immune-diagnostic reagents.
