Involvement of miR-106b in endothelial cells-mediated angiogenesis
10.3969/j.issn.1009-0126.2014.06.022
- VernacularTitle:微小RNA-106b参与内皮细胞介导的血管新生作用机制研究
- Author:
Maimaiti AILIFEIRE
;
Hong CHEN
;
Jingyi REN
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
microRNAs;
transfection;
endothelial cells;
apoptosis;
multigene fami-ly;
activating transcription factor 3
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2014;(6):633-636
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether miR-106b is involved in endothelial cells-mediated angio-genesis .Methods miR-106b cultured and transfected with endothelial cells was divided into miR-106b group ,blank control group and positive control group .RNA was extracted from miR-106b .The transfection efficiency was confirmed by inverse transcription .Endothelial cell tubes formed in matrigel were observed .Apoptosis of transfected miR-106b was assayed by TUNEL assay .Target genes of miR-106b were detected .Expressions of miR-106b and candidate genes were detected by RT-PCR and Western blot ,respectively .Results The number of endothe-lial cell tubes formed in matrigel was significantly less in miR-106b group than in blank control group and positive control group .The ratio of formed tubes ,signal transduction and mRNA ex-pression level were significantly lower in miR-106b group than in positive control group ( P<0.05 ,P<0.01) .No significant difference was found in apoptosis of transfected miR-106b among the 3 groups (1.19% vs 3 .39% ,P>0 .05) .Conclusion miR-106b inhibits endothelial cells-medi-ated angiogenesis by down-regulating the signal transduction and STAT 3 ,which is not directly re-lated with VEGFA .
