Optimization of fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana
- VernacularTitle:醉鱼草杀螺活性内生真菌LL3026发酵条件优化研究
- Author:
Bangxing HAN
;
Jun CHEN
- Publication Type:Journal Article
- Keywords:
Oncomelania hupensis snail;
Molluscicidal activity;
Endophyte;
LL3026;
Fermentation condition;
Orthogonal experiment
- From:
Chinese Journal of Schistosomiasis Control
2014;(3):352-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the fermentation conditions of molluscicidal endophyte LL3026 from Buddleia lindleyana. Method The medium composition and cultivation conditions were optimized by orthogonal and single factor experiments. Re-sults The experiments showed that the conditions of initial pH 3,fermentation temperature 30℃,volume of liquid 100 ml(250 ml Erlenmeyer flask),and 3D-xylitol 0.5 g/L were optimum,and the molluscicidal activity of the fermentation filtrate reached 95%. After three hatches of cultivation,the predicted values were verified by validation experiments. Conclusion Endophyte LL3026 from Buddleia lindleyana has a good molluscicidal activity after the optimization.
