Effects of adiponectin on mitochondrial damage in septic rats with lung injury
10.3760/cma.j.issn.0254-1416.2014.03.028
- VernacularTitle:脂联素对脓毒症大鼠肺损伤时线粒体损伤的影响
- Author:
Hongguang BAO
;
Mengmeng CAI
;
Jianping YANG
;
Yanna SI
;
Liu HAN
;
Tao SHI
;
Hongyu WANG
;
Xinyi XIE
;
Yajie XU
- Publication Type:Journal Article
- Keywords:
Adiponectin;
Sepsis;
Respiratory distress syndrome,adult;
Mitochondria
- From:
Chinese Journal of Anesthesiology
2014;(3):356-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of adiponectin on the mitochondrial damage in septic rats with lung injury .Methods Sixty male Wistar rats ,aged 7-8 weeks ,weighing 180-220 g ,were randomly divided into 3 groups (n=20 each) using a random number table :sham operation group (S group) ,sepsis group (SEP group) and adiponectin group (APN group) .The animals were anesthetized with intraperitoneal 2% pentobarbital sodium 50 mg/kg .Sepsis was produced by cecum ligation and puncture (CLP) .In APN groups ,gene recombinant adiponectin 6 mg/kg was injected intraperitoneally at 12 h after CLP .At 24 h after the operation ,10 rats from each group were chosen and sacrificed ,and lungs were removed for detection of interleukin-6 (IL-6 ) and high-mobility group box-1 (HMGB1 ) contents . The mitochondria of lung samples were isolated for measurement of the malondialdehyde (MDA ) content and degree of mitochondrial swelling and mitochondrial activity . The survival rates within 7 days after operation were recorded in the left 10 rats in each group .Results Compared with S group , IL-6 and HMGB1 contents in lung tissues and MDA content in the mitochondria were significantly increased ,the degree of mitochondrial swelling was increased , and the mitochondrial activity and survival rates within 7 days after operation were decreased in SEP group ( P<0.05 ) . Compared with SEP group , IL-6 and HMGB1 contents in lung tissues and MDA content in the mitochondria were significantly decreased ,the degree of mitochondrial swelling was reduced ,and the mitochondrial activity and survival rates within 7 days after operation were increased in APN groups ( P< 0.05 ) .Conclusion Adiponectin can attenuate lung injury and raise the survival rates in septic rats ,and reduction of mitochondrial damage in lung tissues is involved in the mechanism .
