T-2 toxin inhibits mitochondrial function of differentiated murine embryonic stem cells
10.3867/j.issn.1000-3002.2014.03.018
- VernacularTitle:T-2毒素对小鼠胚胎干细胞线粒体功能的抑制作用
- Author:
Haiqin FANG
;
Lizhong LI
;
Zengming ZHAO
;
Jun HE
;
Jun ZHAO
;
Rong YANG
;
Xue GENG
;
Shuangqing PENG
- Publication Type:Journal Article
- Keywords:
T-2 toxin;
toxic actions;
e mbryonic ste m cells;
mitochondrial;
cell differentiation
- From:
Chinese Journal of Pharmacology and Toxicology
2014;(3):415-420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE ToexplorethepossiblemechanismoractiontargetsofT-2toxinembryo toxicity by observing the effect of T-2 toxin on mitochondrial function of differentiated murine e mbryonic stemcells(mESCs).METHODS Duringdifferentiationat24,72and120h,ESCswereexposedto T-2 toxin 0.5 μg·L-1 .Meanwhile,mESCs were pre-treated with antioxidant Trolox (200 μmol·L-1 )for 30 min and exposed to T-2 toxin (0.5 μg·L-1 )for 72 h.The mitochondrial ultrasture of differentiated mESCs was observed under a transi mission electrical microscope (TEM).The differentiated ESC mito-chondrial function,including respiratory control ratio (RCR),ATP synthase activity and mitochondrial membranepotential(MMP),wasmeasuredat144hafterdifferentiation.RESULTS Significant decrease of the mitochondrial number,deformation of mitochondrial structure,and lack of complete mito-chodrial crest were observed through TEM in the groups of T-2 toxin exposed for 72 and 1 20 h,respec-tively.Compared with the normal control group,RCR declined by 49.5% and 55.1%,ATP synthase activity decreased by 84.9% and 89.3%,and MMP decreased by 23.2% and 35.2% in T-2 toxin 0.5 μg·L-1 exposure 72 and 1 20 h group,respectively.However,the inhibition of mitochondrial function by T-2 toxin in differentiated mESCs recovered significantly in the presence of the antioxidant Trolox. CONCLUSION T-2toxininducesoxidativestressandinhibitsmESCsmitochondrialfunctionindifferenti-ated mESCs,and ROS-induced mitochondrial malfunction plays an i mportant role in T-2 toxin e mbryonic toxicity mechanis m.
