Effect of EGF on angiogenesis induced by adipose-derived stem cells
10.3760/cma.j.issn.1671-0290.2014.03.015
- VernacularTitle:表皮生长因子刺激对脂肪干细胞促血管生成作用的影响
- Author:
Qiang LI
;
Changbo TAO
;
Xueyang LI
;
Zhibing MA
;
Jing WANG
;
Peisheng JIN
- Publication Type:Journal Article
- Keywords:
Adipose-derived stem cells (ADSCs);
Epidermal growth factor (EGF);
Angiogenesis;
Co-culture;
Tube formation
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2014;20(3):210-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of epidermal growth factor (EGF) on tube formation of HUVEC induced by the secretion of angiogenesis factors of adipose-derived stem cells (ADSCs).Methods ADSCs were primarily cultured by enzyme digestion method.The flow cytomertry was performed to detect the expression of cell surface marker.ELISA was used to detect the expression of VEGF,HGF,and SDF-1 after given different doses of EGF.Tube formation assay was used to examine the effect of EGF on the tube formation induced by ADSCs.Results ADSCs were successfully isolated and cultured from human liposuction tissue and specific markers were expressed on ADSCs.EGF promoted the secretion of angiogenesis factors VEGF,HGF,and SDF-1,which were secreted by ADSCs.EGF pretreatment increased the ability of tube formation of HUVECs induced by ADSCs.Conclusions ADSCs induce the secretion of angiogenesis factors in vitro,and thus increase the ability of tube formation of HUVECs.EGF promotes the secretion ability of ADSCs,and the best concentration is 15 mg/L.
