Effect of calcitonin on glutamate release in rat midbrain periaqueductal gray matter
10.3760/cma.j.issn.1000-6699.2014.06.016
- VernacularTitle:降钙素对大鼠中脑导水管周围灰质谷氨酸释放的影响
- Author:
Haiying LI
;
Zhi XIAO
;
Shufa LI
;
Fei XIANG
;
Qing WANG
;
Hui JIANG
- Publication Type:Journal Article
- Keywords:
Calcitonin;
Periaqueductal grey;
Neuron;
Glutamate;
High-performance liquid chromatography
- From:
Chinese Journal of Endocrinology and Metabolism
2014;30(6):511-515
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of calcitonin(CT) in glutamate release from primary cultured neurons of midbrain periaqueductal gray matter(PAG).Methods Primary dissociated culture of PAG neurons was prepared from neonatal Sprague-Dawley (SD) rats.The cultured cells were divided into 4 groups:control group,salmon calcitonin (sCT) group,CT antagonist group (sCT8-32),and protein kinase C (PKC) inhibitor chelerythrine(Che) group.Each group was further divided into subgroups representing low,middle,and high levels of drugs.Glutamate release from the cultured PAG neurons evoked by sCT and/or other interfering factors was detected by using high-performance liquid chromatography (HPLC) assay.Results (1) Compared with the control group,sCT group yielded a time-dependent and concentration-dependent glutamate release from the cultured PAG neurons,and the most effective concentration of sCT was 20 nmol/L(P＜0.01).(2) sCT8-32,a selective antagonist of CT receptor,significantly reversed the effect of 20 nmol/L sCT on glutamate release from cultured PAG neurons,and the most effective concentration was 100nmol/L sCT8-32 (P＜0.01).(3) Incubation of the cultured neurons with Che inhibited the glutamate release from cultured PAG neurons evoked by 20 nmol/L sCT,and 100 μmol/L Che was most effective(P＜0.01).Conclusion CT receptors participates in the glutamate release from PAG neurons in which intracellular protein kinase C signaling pathway is involved.
