Interferon-γ up-regulates expressions of indoleamine 2,3-dioxygenase and tryptophanyl-tRNA-synthetase in human thyrocytes
10.3760/cma.j.issn.1000-6699.2014.06.014
- VernacularTitle:γ-干扰素诱导人甲状腺细胞产生吲哚胺2,3-双加氧酶和色氨酰-tRNA合成酶
- Author:
Xiaofang ZHANG
;
Yicheng QI
;
Qianwei ZHANG
;
Fengjiao HUANG
;
Dongping LIN
;
Guang NING
;
Shu WANG
- Publication Type:Journal Article
- Keywords:
Interferon-γ;
Graves' disease;
Thyrocytes;
Indoleamine 2,3-dioxygenase;
Tryptophanyl-tRNA-synthetase
- From:
Chinese Journal of Endocrinology and Metabolism
2014;30(6):503-506
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of interferon-γ(IFN-γ) on the expression of indoleamine 2,3-dioxygenase(IDO),and tryptophanyl-tRNA-synthetase (TTS) in thyrocytes; and to study the relevant immunopathological significance in Graves' disease.Methods The expressions of IDO and TTS genes in IFN-γ stimulated Nthy-ori3-1 cell line and human thyrocytes,as well as in human thyroid tissues were determined by realtime quantitative PCR.Results IDO and TTS genes were expressed slightly in both Nthy-ori3-1 cell line and human thyrocytes,and were significantly up-regulated after IFN-γ stimulation(P＜0.01).Compared to healthy controls,TTS mRNA level was higher in thyroid tissues of patients with Graves' disease (P =0.018 2),while IDO mRNA level showed no difference,but was notably correlated with IFN-γ mRNA level (R2 =0.716,P =0.002).Conclusion In the early stage of Graves' disease,thyrocytes may decompose local tryptophan by enhancing the expression of IDO and TTS under IFN-γstimulation,thus inhibit auto-reactive function of lymphocytes and balance excessive autoimmune reaction.
