Expression of RASIP1 in hepatocellular carcinoma and its role in suppressing invasion
10.3760/cma.j.issn.1007-8118.2014.05.006
- VernacularTitle:Ras-相互作用蛋白1在肝细胞癌中的表达及其侵袭抑制作用
- Author:
Dipeng OU
;
Defu WU
;
Hao YANG
;
Dong HUA
;
Lianyue YANG
- Publication Type:Journal Article
- Keywords:
Gene,RASIP1;
Ras-interacting protein 1;
Hepatocellular carcinoma;
Invasion;
Recurrence and metastasis
- From:
Chinese Journal of Hepatobiliary Surgery
2014;20(5):342-345
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression levels of RAS-interacting protein 1 (RASIP1) mRNA and protein in hepatocellular carcinoma (HCC) tissues and its cell lines,and to analyze the relationship between RASIP1 and tumorigenesis and progression of hepatocellular carcinoma.Methods The expression levels of RASIP1 mRNA and protein in 29 hepatocellular carcinoma tissues and the corresponding adjacent non-cancer liver tissues (ANLTs),as well as those in the HCC cell lines such as LO2,HEPG2,MHCC97-H and HCCLM3 were detected using real-time PCR and western blot.Results The RASIP1 expression levels decreased significantly in HCC tissues when compared with the corresponding ANLTs; The expression levels of RASIP1 mRNA and protein in LO2 were significantly higher than those in other HCC cell lines (P ＜ 0.05) ; The expression levels of RASIP1 mRNA and protein in MHCC97-H and HCCLM3 were significantly lower than those in HepG2 (P ＜ 0.05).Conclusions HCC tissues had lower expression than those in ANLTs.On analyzing the RASIP1 levels of HCC tissues and its cell lines,we speculated that RASIP1 might suppress recurrence and metastasis of HCC.
