Experimental study of platelet derived growth factor expression in small bowel transplantation of rats

VernacularTitle:血小板源性生长因子在小肠移植慢性排斥反应中表达的实验研究
Author: Xiaoshi JIN ; Zhengwei JIAO ; Aimin ZHANG ; Shujie CHENG ; Tong LIU ; Pengzhi WANG
Publication Type:Journal Article
Keywords: Platelet derived growth factor; Small bowel transplantation; Chronic rejection
From: Clinical Medicine of China 2013;29(z1):5-7
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of platelet derived growth factor(PDGF) in small bowel transplantation of rats.Methods Isogeneic and allogeneic small bowel transplantation were performed in rats by microsurgical technology.All rats were divided into two groups:isogeneic control group and allogeneic test group.Transplanted tissues were test on 7th,28th and 90th after surgery.Positioning using immunohistochemical method the expression of PDGF.Real time PCR and immunohistochemical staining were also performed to detect the expression of PDGF.Results The unique feature including intestinal graft fibrosis was showed in tissues.Immunohistochemistry results showed PDGF expression was higher in intestinal glands.PDGF mRNA levels in transplanted tissues showed a gradual upward trend,and the top levels is in POD90.Conclusion PDGF expression was significantly higher in the late of intestinal transplantation,which showed an guideline for chronic rejection of intestinal transplantation.