Subcellular localization of the transmembrane inner ear (Tmie) protein in a stable Tmie-expressing cell line.
10.5625/lar.2011.27.4.339
- Author:
Sankarapandian KARUPPASAMY
1
;
Yoon Yi NAM
;
Harry JUNG
;
Byoungkwon PARK
;
Hyung Joo KWON
;
Jun Gyo SUH
Author Information
1. Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Korea. jgsuh@hallym.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Transmembrane inner ear protein;
subcellular localization;
hearing loss disorder;
HEK293
- MeSH:
Blotting, Western;
Cell Line;
Cytoplasm;
Deafness;
Ear, Inner;
Hearing Loss;
Membranes
- From:Laboratory Animal Research
2011;27(4):339-342
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mutations in the transmembrane inner ear (Tmie) gene, which encodes the Tmie protein, have been attributed to deafness autosomal recessive 6 (DFNB6), an autosomal nonsyndromic recessive hearing loss disorder. Although the Tmie gene was identified a few years ago, little is known about subcellular localization of the Tmie protein. In order to address this, we developed a stable cell line expressing Tmie protein. The expression of Myc-tagged Tmie protein was confirmed by Western blot analysis using an anti-Myc antibody and localization of the Tmie protein was confirmed by immunostaining, using the anti-Myc antibody as well as the anti-tmie antibody. Our study demonstrates that the Tmie protein is localized mostly in the cellular membrane and to a lesser extent in cytoplasm. These results suggest that our Tmie expressing stable cell line provides a suitable in vitro model to explore Tmie synthesis and functions.
