Genetype distribution of mycoplasma pneumoniae by denaturing high-performance liquid chromatography

VernacularTitle:变性高效液相色谱分析肺炎支原体基因类型研究
Author: Zhenyao DING ; Hongwei LI ; Meili GUO ; Quanzhong FANG ; Yulong YU
Publication Type:Journal Article
Keywords: Denaturing high-performance liquid chromatography; Mycoplasma pneumoniae; Genetype
From: Chinese Pediatric Emergency Medicine 2014;21(6):366-368
CountryChina
Language:Chinese
Abstract: Objective To investigate the genetype distribution of mycoplasma pneumoniae(MP) by denaturing high-performance liquid chromatography(DHPLC).Methods A total of 300 cases nasopharyngeal aspirate were collected from our hospital.The MP genes of standard strains and clinical specimens isolates were amplified by PCR followed by DHPLC and genetype determination.Results A total of 110 cases were positive after 24 hours fermentation from 300 cases with pharyngeal swab.By the specific primers of MP-129,MP-FH standard strain and specimens,2 280 bp and 2 580 bp gene fragments were made out respectively.One hundred and ten strains of clinical isolates were detected by DHPLC.One hundred and seven strains of P1-Ⅰ were 1b subtype,3 were type P1-Ⅱ which were all 2a subtype.Conclusion The genetype of MP infection in children from our hospital is P1-Ⅰ,1b subtype by using DHPLC technology.