The expression and significance of hypoxia-inducible factor-1α in neonatal rats with hypoxic pulmonary hypertension
10.3760/cma.j.issn.1673-4408.2014.03.024
- VernacularTitle:低氧性肺动脉高压大鼠低氧诱导因子-1α表达及其意义
- Author:
Jun LI
;
Jianhua FU
;
Xindong XUE
;
Yanna DU
;
Haiqin GENG
- Publication Type:Journal Article
- Keywords:
Hypoxia-inducible factor-1α;
Vascular remodeling;
Pulmonary hypertension;
Neonatal rats
- From:
International Journal of Pediatrics
2014;41(3):305-309
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic expression from gene and protein levels of hypoxia inducible factor-1α(HIF-1α) during the development of hypoxic pulmonary hypertension (HPH) in neonatal rats.Methods A neonatal rat model of HPH was established,normal neonatal rats were enrolled as the control group.At the 3th 、7th、10th and 14th days,we measured the mRVSP through catheterization of right ventricule,collected hearts to figure out the right ventricular hypertrophy index(RVHI),evaluated vascular remodeling by the percentage of medial thickness to outer diameter of the small pulmonary arteries (MT％) and the percentage of medial cross section on area to the total cross section area of the pulmonary small arteries (MA％),observed the expression of HIF-1α by immunochemistry,and measured the expression of HIF-1α in mRNA and protein by RT-PCR and Western blot respectively.Results The mRVSP increased at the 3th day,and sustained until the 14th day (P ＜ 0.01).RVHI MT％ and MA％ increased at the 7th day,and sustained until the 14th day (P ＜0.01).HIF-1α mainly expressed in endothelium and smooth muscle cells in the CHPH group and the HIF-1αmRNA increased significantly on the 3th and 7th days (P ＜ 0.05),and the HIF-1α protein increased significantly on the 7th、10th and 14th days in the CHPH group compared with the control group(P ＜ 0.01).Conclusion The mRVSP increased at the early stage after hypoxic exposure in neonatal rats,followed by vascular remodeling and right ventricular hypertrophy.Both mRNA and protein levels of HIF-1α sustained higher than control group during the vascular remodeling stage,indicating that HIF-1α might be a important factor contributing to the vascular remodeling.
