The level and clinical significance of serum anti-lysosomal associated membrane protein-2 antibody in patients with ANCA-associated glomerulonephritis
10.3760/cma.j.issn.1001-7097.2014.07.003
- VernacularTitle:溶酶体相关膜蛋白2抗体在ANCA相关性肾炎患者血清中的表达水平及意义
- Author:
Xi CHEN
;
Zhidong HU
- Publication Type:Journal Article
- Keywords:
Antibodies;
Lysosomal-associated membrane protein-2;
Antibodies,antineutrophil cytoplasmic;
ANCA-associated glomerulonephritis
- From:
Chinese Journal of Nephrology
2014;30(7):494-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of the serum anti-lysosome associated membrane protein 2 (LAMP-2) antibody levels and anti-neutrophil cytoplasmic antibodies (ANCA)-associated glomerulonephritis.Methods Thirty-three patients with new onset ANCA-associated glomerulonephritis and thirty healthy controls were enrolled.ANCA detection was performed using indirect immunofluorescence (IIF).Enzyme-linked immunosorbent assay (ELISA) was used to detect myeloperoxidase (MPO),proteinase-3 (PR3) and other ANCA-associated antibodies including LAMP-2.The cut-off value of the serum anti-LAMP-2 antibody was determined by a receiver operating characteristic (ROC) curve.Results The serum levels of anti-LAMP-2 antibody in new onset ANCA-associated glomerulonephritis patients were significantly higher than remission stage ANCA-associated glomerulonephritis patients and healthy controls (P ＜ 0.05).The serum levels of anti-LAMP-2 antibody showed no visible difference between the remission ANCA-associated glomerulonephritis patients and healthy controls (P ＞ 0.05).The levels of anti-LAMP-2 antibody showed a strong positive correlation with ESR,Scr,BUN,proteinuria,crescent proportion and Birmingham vasculitis activity score (BVAS) and a negative correlation with Ccr,Hb and Alb.Conclusions Anti-LAMP-2 antibody is correlated with the activity of ANCA-associated glomerulonephritis and the severity of renal damage.It may be a useful indicator on the activity of ANCA-associated glomerulonephritis.
